goat monoclonal r d systems cat  (R&D Systems)

Journal: Foods

Article Title: The Combined Antioxidant Effects of N-Acetylcysteine, Vitamin D3, and Glutathione from the Intestinal–Neuronal In Vitro Model

doi: 10.3390/foods13050774

Figure Lengend Snippet: Hepatic activity on the 3D in vitro model stimulated with the combination of NAC 12 mM in the presence of VitD3 100 nM and Glutathione 5 mM and compared to LA 50 µM. ( a ) Cell viability measured by MTT test; ( b ) TNFα measurement by ELISA kit; ( c ) NF-Kb measurement by ELISA kit; ( d ) NAC dosage performed with spectrophotometry technique. Data are expressed as means ± SD (%) of five independent experiments performed in triplicate and normalized to control values (the 0% line). In ( a – c ), * p < 0.05 vs. control; α p < 0.05 vs. Lipoic Acid 50 µM; β p < 0.05 vs. VitD3; γ p < 0.05 vs. glutathione; δ p < 0.05 vs. NAC 12 mM. In ( d ), * p < 0.05 vs. control and the bars p < 0.05 vs. NAC 12 mM.

Article Snippet: The Human Phospho-Src (Y419) Duo set ELISA Kit (R&D Systems, Minneapolis, MN, USA) was used to measure phospho-Src in HepG2 cell lysate according to the manufacturer’s instructions.

Techniques: Activity Assay, In Vitro, Enzyme-linked Immunosorbent Assay, Spectrophotometry, Control

Journal: Foods

Article Title: The Combined Antioxidant Effects of N-Acetylcysteine, Vitamin D3, and Glutathione from the Intestinal–Neuronal In Vitro Model

doi: 10.3390/foods13050774

Figure Lengend Snippet: Analysis of intracellular markers involved in hepatic homeostasis on a 3D in vitro liver model after 24 h. ( a ) CYP1A2, ( b ) CYP3A4, ( c ) SRC, and ( d ) ERK/MAPK activities measured by ELISA kit. Data are mean ± SD of five independent experiments performed in triplicates and expressed as percentages (%) vs. control values (0% line). * p < 0.05 vs. control; α p < 0.05 vs. Lipoic Acid 50 µM; β p < 0.05 vs. VitD3; γ p < 0.05 vs. glutathione; δ p < 0.05 vs. NAC 12 mM.

Article Snippet: The Human Phospho-Src (Y419) Duo set ELISA Kit (R&D Systems, Minneapolis, MN, USA) was used to measure phospho-Src in HepG2 cell lysate according to the manufacturer’s instructions.

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Control

Journal: Foods

Article Title: The Combined Antioxidant Effects of N-Acetylcysteine, Vitamin D3, and Glutathione from the Intestinal–Neuronal In Vitro Model

doi: 10.3390/foods13050774

Figure Lengend Snippet: Analysis of the beneficial effects on CCF-STTG1 cells. ( a ) NAC quantification by spectrophotometry technique and ( b ) cell viability measured by MTT test in a study from 1 h to 6 h; ( c ) ROS production analysis measured through cytochrome C reduction in CCFTG cells after 24 h; ( d ) TNFα and ( e ) IL1- β activities measured by ELISA kit on CCFTG cells after 24 h. Data are mean ± SD of five independent experiments performed in triplicates vs. control values (0% line). * p < 0.05 vs. control; α p < 0.05 vs. Lipoic Acid 50 µM; β p < 0.05 vs. VitD3; γ p < 0.05 vs. glutathione; δ p < 0.05 vs. NAC 12 mM.

Article Snippet: The Human Phospho-Src (Y419) Duo set ELISA Kit (R&D Systems, Minneapolis, MN, USA) was used to measure phospho-Src in HepG2 cell lysate according to the manufacturer’s instructions.

Techniques: Spectrophotometry, Enzyme-linked Immunosorbent Assay, Control

Journal: Foods

Article Title: The Combined Antioxidant Effects of N-Acetylcysteine, Vitamin D3, and Glutathione from the Intestinal–Neuronal In Vitro Model

doi: 10.3390/foods13050774

Figure Lengend Snippet: Analysis of biomarkers in CCF-STTG1 cells. ( a ) pTAU activity and ( b ) APP activity measured by ELISA kit on CCFTG cells after 24 h. Data are mean ± SD of five independent experiments performed in triplicates vs. control values (0% line). * p < 0.05 vs. control; α p < 0.05 vs. Lipoic Acid 50 µM; β p < 0.05 vs. VitD3; γ p < 0.05 vs. glutathione; δ p < 0.05 vs. NAC 12 mM.

Article Snippet: The Human Phospho-Src (Y419) Duo set ELISA Kit (R&D Systems, Minneapolis, MN, USA) was used to measure phospho-Src in HepG2 cell lysate according to the manufacturer’s instructions.

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: Foods

Article Title: The Combined Antioxidant Effects of N-Acetylcysteine, Vitamin D3, and Glutathione from the Intestinal–Neuronal In Vitro Model

doi: 10.3390/foods13050774

Figure Lengend Snippet: Effects of the substances on PNS in vitro model after 24 h. ( a ) Cell viability measured by the MTT test; ( b ) TNFα and ( c ) IL1- β activities analysed by ELISA kit; ( d ) p75 activity performed by ELISA kit; ( e ) NAC concentration determined by spectrophotometry technique. In all panels, VitD3 100 nM and Glut 5 mM are also reported. Data are mean ± SD of five independent experiments performed in triplicates vs. control values (0% line). * p < 0.05 vs. control; α p < 0.05 vs. Lipoic Acid 50 µM; β p < 0.05 vs. VitD3; γ p < 0.05 vs. glutathione; δ p < 0.05 vs. NAC 12 mM.

Article Snippet: The Human Phospho-Src (Y419) Duo set ELISA Kit (R&D Systems, Minneapolis, MN, USA) was used to measure phospho-Src in HepG2 cell lysate according to the manufacturer’s instructions.

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Activity Assay, Concentration Assay, Spectrophotometry, Control

Journal: Foods

Article Title: The Combined Antioxidant Effects of N-Acetylcysteine, Vitamin D3, and Glutathione from the Intestinal–Neuronal In Vitro Model

doi: 10.3390/foods13050774

Figure Lengend Snippet: Analysis of Schwann cell activity after 24 h. ( a ) p75; ( b ) MPZ; ( c ) NRG1; ( d ) ERB3 activities analysed by ELISA kit. Data are mean ± SD of five independent experiments performed in triplicates vs. control values (0% line). * p < 0.05 vs. control; α p < 0.05 vs. Lipoic Acid 50 µM; β p < 0.05 vs. VitD3; γ p < 0.05 vs. glutathione; δ p < 0.05 vs. NAC 12 mM.

Article Snippet: The Human Phospho-Src (Y419) Duo set ELISA Kit (R&D Systems, Minneapolis, MN, USA) was used to measure phospho-Src in HepG2 cell lysate according to the manufacturer’s instructions.

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Control